• Product Name: Fluoroskan Ascent FL Fluorescence/Chemiluminescence Analyzer
• Order number: tlyon000389
• Brand Name: Thermo, USA

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The Fluoroskan Ascent FL series fluorescence/chemiluminescence analyzer combines fluorescence and chemiluminescence technologies, capable of detecting fast and slow kinetic chemiluminescence reactions, and possessing all the excellent performance of the Fluoroskan Ascent fluorescence luminescence analyzer. Combined with the powerful Ascent software, Luminoskan Ascent FL can perform excellently in scientific research, clinical, and industrial applications.

Product Features

2-in-1 fluorescence/chemiluminescence analysis technology with excellent performance

The latest optical system has high sensitivity

User friendly Ascent software simplifies fluorescence and chemiluminescence applications

Unique injection system applied to rapid (flash kinetic) luminescent reactions

● Suitable for multiple board types -1 hole -384 holes

● Can be used in conjunction with automated robotic arms to process high-throughput samples

Excellent performance

The Fluoroskan Ascent FL system is capable of reading various plate types such as 1-384 well plates. The efficient optical path system enables the system to detect fluorescein as low as 2 fmol per well, with a linear measurement range of up to 7 orders of magnitude or more. For the convenience of application, the system can be configured with up to 3 automatic samplers. The automatic sampler can accurately add reagents with an adjustable range of 5-1000ul. Sample addition and detection can be performed simultaneously, meeting the needs of rapid chemiluminescence reactions, fluorescence Ca2+detection, and other rapid reactions. For tests that require precise temperature control, the system provides a standard incubator. In addition, the diameter and speed of the standard circular oscillator can be adjusted to ensure effective mixing and accelerate reaction time.

IQ/OQ/PQ

Provide Fluoroskan Ascent FL IQ/OQ/PQ system quality certification documents, and users can complete instrument quality certification in the laboratory according to the document instructions. For detailed information, please contact Thermo Fisher Scientific.

Easy to be used in conjunction with automatic machinery

The Fluoroskan Ascent FL system can be used in conjunction with various automated machinery to meet the needs of high-throughput applications. Optional enzyme-linked immunosorbent assay (ELISA) plate holder for easy integration with different models of robotic arms. The function of Ascent software that can be used in conjunction with automatic and HIS/LIMS systems



High performance optical system

The optical system of Fluoroskan Ascent FL generates highly concentrated light beams through a mirror and prism system, eliminating cross effects. Two optional beam systems are available for reading 1-96 well plates and 384 well plates. The general beam diameter is 3mm, and the narrow beam diameter is 1.5mm. High sensitivity is a unique feature of fiber free optical systems and is necessary for chemiluminescence research. By adding filters, the system can be flexibly applied to fluorescence research.


Powerful software with powerful functions

Ascent software is specifically designed for fluorescence and chemiluminescence reactions, and the two detection methods can be compared in the same experiment. Users can set experimental methods or use pre-set experimental methods for easy experimentation.

Fluorescent DNA quantification

Various fluorescence methods can be used for DNA quantification. PicoGreen is a highly sensitive dye used for DNA endpoint detection. The system can automatically calculate DNA concentration, and the standard curve is smoothed using linear regression. The detailed analysis, including standard curves and calculation results, is included in the final report.

Study on the bioluminescence of jellyfish luminescent protein (Aequorin)

Jellyfish luminescent proteins and their derivatives can serve as highly sensitive markers for application in bioluminescence immunoassay and hybridization analysis. During these processes, the rapid response of jellyfish luminescent proteins requires rapid detection of the signals generated. The Fluoroskan Ascent FL system and Ascent software support simultaneous sample addition and reading, ensuring that the jellyfish luminescent protein signal can be detected from the beginning.

Technical Parameter

Enzyme labeled plate types 1, 6, 12, 24, 48, 96, and 384 well plates (such as Terasaki plates)

It can also be programmed for specific board types. The maximum size is 90mm × 134mm × 25mm

The minimum detection time for 96 well enzyme-linked immunosorbent assay plates is 15 seconds

Rocking bed with rails, speed 60-1200rpm, diameter 1-50mm

Injector 1-3 Sample Dispensers

Processing volume 5-1000ul, 1ul increment

Injection speed 25 seconds/plate (96 well plate, 5ul/well)

Incubation temperature range: room temperature+3 ℃ -453 ℃

Fluorescent light path system

Light source quartz halogen lamp

PMT detector

Excitation wavelength 320nm-700nm

Emission wavelength 360nm-800nm

High quality filter, the excitation light filter wheel can hold up to 8 filters, and the emission light filter wheel can hold up to 6 filters

Excitation light filter 320 nm，355nm，390nm，430nm，440nm，444nm，485nm，530nm，544nm，578nm，584nm，646nm

Emission light filter 405 nm，460nm，485nm，510nm，518nm，520nm，527nm，538nm，555nm，590nm，590nm，604nm，612nm，620nm，678nm，680nm

Theoretical sensitivity of 2fmol Fluresein/well, using a black 96 well plate

Linear range>9 orders of magnitude (9 decks)

Chemiluminescence optical path system

PMT detector

Spectral range 270nm-670nm

Theoretical sensitivity of 5fmolATP/well, (using Thermoelectric ATP detection kit)

Linear range>9 orders of magnitude (9 decks)

Except for the filters already included in the host, other filters need to be ordered separately; In addition to the standard filters mentioned above, various non conventional filters can also be customized

basic filter

Ordering Information

Cat.No. Description

5210450 Fluoroskan Ascent FL Fluorescence/Chemiluminescence Analyzer

5210460 Fluoroskan Ascent FL Fluorescence/Chemiluminescence Analyzer with 1 Injector

5210230 2nd and 3rd Injectors

5210290 wide spectral range PMT, 360-800nm

Including software and Ex355nm/Em460nm; Ex485/Em538nm two pairs of filters



Fluoroskan Ascent FL series fluorescence analyzer application areas:

● ATP detection

Immunoassay (lmmunoassays)

Intracellular Ca2+detection

● Reporter gene assay

Hybridization assay

● Cell proliferation

Cytotoxicity

● Cell adhesion

Bacterial quantification

Enzyme activity analysis

Phagocytosis

DNA Quantification

Molecular beacon assay

FRET assay

BRET and BRET2 assay

Oligonucleotide assay